UP AQUA CO2 regulator designed to easily connect. Simply tighten the regulator clockwise direction by hand,without the need of any tool. / To release simple regulator from the CO2 bottle, to be sure the valve of the bottle is close, then release remnant gas pressure from the regulator, then unscrew from the bottle./ Do not unscrew the regulator before remnant gas pressure released, that cause damage of the regulator 0-rig. / For 4/6mm co2 tube.// All kind of CO2 Cylinder as a high pressure Cylinder contain with 3-4 kg / cm2 liquid state CO2, UP Aqua CO2 regulator specially design for use in high pressure system.